论文部分内容阅读
用两株人胃腺癌细胞系（ＧＣ－８０３和ＳＧＣ－７９０１）接种于裸小鼠皮下，形成初代移植瘤后鼠间传代，观察和比较了两株胃癌细胞系初代和子代移植瘤的移植成功率、生长特性和组织病理变化。结果显示：两株胃癌细胞系均能形成裸鼠皮下移植瘤，鼠间传代目前已至第１０代，总移植成功率达１００％，尚未发现移植瘤自发消退现象。比较两组移植瘤的生长速度，发现ＳＧＣ－７９０１组比ＧＣ－８０３组生长迅速。实验中未观察到裸鼠的主要脏器有转移灶形成。组织学检查表明两组移植瘤均为低分化腺癌结构，染色体分析显示两株移植瘤均具有人类恶性肿瘤细胞染色体的特点。该裸鼠移植瘤模型可作为一种有价值的工具用于胃癌防治的研究
Two human gastric adenocarcinoma cell lines (GC-803 and SGC-7901) were inoculated subcutaneously in nude mice to form primary transplanted tumors and then passaged between mice. Observe and compare the successful transplantation of primary and secondary transplanted tumors of two gastric cancer cell lines. Rates, growth characteristics, and histopathological changes. The results showed that both gastric cancer cell lines can form subcutaneous xenografts in nude mice. The passage between mice has reached the 10th generation, and the total transplantation success rate reaches 100%. No spontaneous tumor regression has been found. The growth rate of the transplanted tumors in the two groups was compared and it was found that the SGC-7901 group grew faster than the GC-803 group. No metastasis formation was observed in the primary organs of nude mice. Histological examination showed that the two groups of transplanted tumors were poorly differentiated adenocarcinoma structures. Chromosomal analysis showed that both transplanted tumors had the characteristics of human malignant tumor cells. The nude mouse xenograft model can be used as a valuable tool for the study of gastric cancer prevention and treatment