论文部分内容阅读
目的检测肝癌组织中SnailmRNA和蛋白的表达及其与肝癌侵袭转移的关系。方法采用免疫组织化学方法检测70例肝癌组织及其癌旁组织中Snail蛋白的表达,采用RT-PCR方法检测40例新鲜肝癌组织及其癌旁组织中SnailmRNA的表达,并结合临床病理指标进行分析。结果肝癌组织中Snail的阳性表达率高于癌旁组织中(P<0.05),且癌组织中Snail阳性表达率与包膜侵袭、门脉侵袭及肝内转移情况有关(P<0.05)。肝癌组织中SnailmRNA的阳性表达率高于癌旁组织(P<0.05),且癌组织中SnailmRNA的阳性表达率与包膜侵袭、门脉侵袭情况有关(P<0.05),而与肝内转移情况无关(P>0.05)。结论肝癌的癌变、侵袭、转移可能与Snail蛋白及mRNA的高表达有关,抑制Snail的表达,有望成为治疗肝癌的新靶点。
Objective To detect the expression of Snail mRNA and protein in hepatocellular carcinoma and its relationship with the invasion and metastasis of hepatocellular carcinoma. Methods Immunohistochemistry was used to detect the expression of Snail protein in 70 cases of HCC tissues and their adjacent tissues. The expression of Snail mRNA in 40 cases of HCC tissues and their adjacent tissues was detected by RT-PCR and analyzed with clinicopathological parameters . Results The positive expression rate of Snail in HCC tissues was significantly higher than that in adjacent tissues (P <0.05). The positive rate of Snail in HCC tissues was related to invasion of invasion, portal vein invasion and intrahepatic metastasis (P <0.05). The positive rate of Snail mRNA in HCC tissues was significantly higher than that in paracancer tissues (P <0.05). The positive rate of Snail mRNA in HCC tissues was related to the invasion of invasion and portal vein invasion (P <0.05), but not to the intrahepatic metastasis Not related (P> 0.05). Conclusion The carcinogenesis, invasion and metastasis of HCC may be related to the high expression of Snail protein and mRNA, and the inhibition of Snail expression may be a new target for the treatment of HCC.