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对9例人原发性肝癌,1例癌旁组织,1例正常肝的poly(A)~+RNA进行了分析。用各种癌基因探针作分子杂交,发现在6例原发性肝癌中,有二条增强表达的区带:2.2kb和5.6kb。肝癌组织较正常肝的mRNA在2.2kb处有明显的增强。提示人N-ras基因的转录产物明显增高。癌旁,正常肝中N-ras基因的专一的mRNA很弱或低于检测水平。由于癌的发生是通过癌基因产物发生作用,因此N-ras基因在多数的人原发性肝癌中的表达明显增强,提示了N-ras基因是人原发性肝癌的重要转化基因之一。
Poly (A) ~ + RNA was analyzed in 9 cases of primary liver cancer, 1 case of paracancerous tissue and 1 case of normal liver. Using a variety of oncogene probes for molecular hybridization, two of the six primary liver cancers were found to have enhanced expression bands: 2.2 kb and 5.6 kb. Histopathological liver tissue mRNA was significantly increased at 2.2kb. Suggesting that the transcriptional product of human N-ras gene was significantly increased. Specific mRNAs of N-ras genes in para-cancerous and normal liver are very weak or below detection level. Since carcinogenesis occurs through oncogene products, the expression of N-ras gene in most human primary hepatocellular carcinomas is significantly increased, suggesting that N-ras gene is one of the important transforming genes in human primary hepatocellular carcinoma.