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目的检测慢性乙型肝炎及肝炎肝硬化患者血清中转化生长因子β-1(TGFβ-1)、瘦素(Leptin)的含量并分析其与透明质酸(HA)、层黏连蛋白(LN)和前胶原蛋白Ⅲ(PCⅢ)等肝纤维化指标的相关性。方法分别用放免法及酶联免疫法测定109例慢性乙肝及肝炎肝硬化患者血清TGFβ-1、Leptin和HA、LN、PCⅢ的浓度。结果慢性乙肝重度及肝炎肝硬化组患者血清TGFβ-1水平明显高于慢性乙肝轻、中度及正常对照组(P均<0.05);且与HA、LN、PCⅢ等指标存在明显的正相关;而慢性乙肝轻、中度组血清TGFβ-1水平与正常对照组无显著性差异(P>0.05)。慢性乙肝及肝炎肝硬化组患者血清Leptin水平高于正常对照组,但差异无显著性(P>0.05);且与TGFβ-1、HA、LN、PCⅢ等指标无相关关系。结论TGFβ-1参与慢性乙型肝炎、肝硬化的进程,可作为诊断肝纤维化有用的血清学指标;慢性乙肝及肝炎肝硬化组患者血清leptin水平升高,但并未参与肝硬化的进程。
Objective To detect the levels of serum transforming growth factor β-1 (TGFβ-1) and leptin in patients with chronic hepatitis B and hepatitis cirrhosis and analyze the relationship between them with the levels of hyaluronic acid (HA), laminin (LN) And procollagen Ⅲ (PC Ⅲ) and other indicators of liver fibrosis. Methods Serum levels of TGFβ-1, Leptin, HA, LN and PCⅢ in 109 patients with chronic hepatitis B and hepatitis cirrhosis were determined by radioimmunoassay and enzyme-linked immunosorbent assay respectively. Results Serum levels of TGFβ-1 in patients with chronic hepatitis B and hepatitis cirrhosis were significantly higher than those in mild, moderate and chronic hepatitis B patients (all P <0.05), and were positively correlated with HA, LN and PCⅢ. However, there was no significant difference between the serum levels of TGFβ-1 in chronic mild hepatitis B and normal controls (P> 0.05). Serum Leptin levels in patients with chronic hepatitis B and hepatitis cirrhosis were significantly higher than those in the normal control group (P> 0.05). There was no correlation between serum leptin and TGFβ-1, HA, LN and PCⅢ. Conclusion The involvement of TGFβ-1 in the pathogenesis of chronic hepatitis B and cirrhosis may be a useful serological marker for diagnosis of hepatic fibrosis. Serum leptin levels are elevated in patients with chronic hepatitis B and hepatitis cirrhosis but not in cirrhosis.