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目的:将治糜灵栓剂(《中国药典》2010版一部)改为治糜灵凝胶剂,建立治糜灵凝胶剂的薄层色谱鉴别方法,为制定其质量控制标准制定依据。方法:参照《中国药典》2010版一部治糜灵栓剂项下黄柏、苦参、儿茶、冰片的薄层色谱鉴别方法,对处方中的主要药味进行定性鉴别。黄柏鉴别中以甲苯-乙酸乙酯-异丙醇-水(6:3:1.5:1.5)为展开剂;苦参鉴别中以甲苯-乙酸乙酯-甲醇(8:3:0.5)为展开剂;儿茶鉴别中以正丁醇-醋酸-水(3:2:1)为展开剂;冰片鉴别中以环己烷-三氯甲烷-乙酸乙酯(9:1:2)为展开剂。结果:薄层色谱上具有黄柏,苦参,儿茶,冰片的鉴别特征,且阴性对照无干扰。结论:色谱斑点清晰,专属性强;该方法操作简便,稳定性、重现性均很好。可作为质量标准的控制依据。证明治糜灵凝胶剂研究的方法可行。
OBJECTIVE: To change the method of treating Mi Ling suppository (“Chinese Pharmacopoeia” 2010 edition) to cure Mi Ling gel, and to establish the TLC identification method for treating Mi Ling gel in order to formulate its quality control standards. Methods: According to the “Chinese Pharmacopoeia” 2010 version of a under the Mi Ling suppository phellodendron, Sophora, catechu, borneol TLC identification method, the prescription of the main flavor qualitative identification. Cortex Phellodendri differential identification with toluene - ethyl acetate - isopropanol - water (6: 3: 1.5: 1.5) as the developing agent; Sophora identification with toluene - ethyl acetate - methanol In the identification of catechuol, n-butanol-acetic acid-water (3: 2: 1) was used as the developing solvent; cyclohexane-trichloromethane-ethyl acetate (9: 1: 2) as the developing solvent in the identification of borneol. Results: The TLC showed cortex phellodendri, Sophora flavescens, catechu, borneol identification characteristics, and the negative control without interference. Conclusion: The chromatographic spots are clear and specific. The method is simple, stable and reproducible. Can be used as the basis of quality control standards. Prove that cure Mi Ling gel research method is feasible.