本文报道14个香蕉品种或品系进行小茎尖离体培养繁殖无病苗。小茎尖培养在改良MS培养基中,附加BA2.0—5.0mg/l,试验结果显示,BA明显促进芽的形成和增殖,随着BA浓度的增高,形成的芽苗数也随着增多。各品种均能诱导丛生芽,但品种间的繁殖率有很大差异。低浓度的Kt或BA有利于诱导生根。培养的试管苗经检验为无病苗。 In this paper, 14 banana cultivars or lines were used to conduct in vitro culture of small shoots without disease-free seedlings. Small shoots were cultured in modified MS medium supplemented with BA 2.0-5.0mg / l. The results showed that BA significantly promoted the formation and proliferation of shoots. With the increase of BA concentration, the number of shoots formed increased . All varieties can induce clustered buds, but there is a big difference between the breed rates. Low concentrations of Kt or BA help induce rooting. The cultured plantlets were tested as vaccine-free.