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目的对采用不同种类的砧木培育出的化橘红药材进行黄酮类成分含量测定,初步探究砧木种类对其黄酮类成分含量的影响。方法以柚皮苷为对照品,利用紫外分光光度法测定各样品中总黄酮含量,以HPLC法同时测定各样品中柚皮苷及野漆树苷含量。结果所建立的可见分光光度法的加样回收率为95.81%,RSD为1.06%。HPLC法同时测定柚皮苷与野漆树苷含量在进样量为1.54~30.80μg和0.07~1.40μg范围内与色谱峰面积积分值呈现良好的线性关系,相关系数分别为0.998,0.997。柚皮苷与野漆树苷的加样回收率分别为98.11%(RSD=3.42%)、98.63%(RSD=2.37%)。不同砧木培育的化橘红黄酮类成分含量差异显著。与凤尾化橘红相比,本地柚砧木凤尾野漆树苷含量与其相近,其他品种均明显低于凤尾化橘红。结论不同种类的砧木对化橘红中黄酮类成分的含量影响较大。非本地柚为砧木芽接后化橘红药材野漆树苷含量显著下降,以本地柚为砧木嫁接凤尾芽苗培育出的化橘红品质较优。
OBJECTIVE To determine the flavone content of Huaxia red medicinal materials cultivated with different kinds of rootstocks and to explore the effect of rootstock species on the flavonoid content. Methods Naringin was used as a reference substance. The content of total flavonoids in each sample was determined by UV spectrophotometry. The contents of naringin and raparadin in each sample were determined simultaneously by HPLC. Results The visible spectrophotometry established recovery was 95.81% with RSD 1.06%. Simultaneous determination of naringin and naringin by HPLC showed a good linear relationship with the chromatographic peak area integral values in the range of 1.54-30.80μg and 0.07-1.40μg with correlation coefficients of 0.998 and 0.997, respectively. The recoveries of naringin and naringin were 98.11% (RSD = 3.42%) and 98.63% (RSD = 2.37%), respectively. The content of Citrus flavonoids cultivated in different rootstocks was significantly different. Compared with Pteridophyta orange, the content of Pomodorovanoside in the local grapefruit rootstock was similar to that of Pteridophyta, while the other varieties were significantly lower than Pteridophyta orange. Conclusion Different kinds of rootstocks have a great influence on the content of flavonoids in the tangerine juice. Non-native grapefruit as rootstock after the orange-huang medicine Radix notoginseng content decreased significantly, with local grapefruit as rootstock grafted phlox sprout nurturing the orange-red quality is better.