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目的 了解慢性乙型肝炎病毒 (HBV)携带者外周血单个核细胞 (PBMC)在体外对抗原诱导的抗体应答反应 .方法 选择 18例无症状 HBV携带者 (ASC)和 18例慢性乙型肝炎患者 (SC) ,同时选择 15例自愿者作正常对照 ,分离PBMC,在体外应用血源乙肝表面抗原 (HBs Ag)和重组乙肝核心抗原 (r HBc Ag)刺激 ,检测相应特异性抗体的产生 .结果 1无症状 HBV携带者和慢性乙型肝炎患者均有不同程度的抗体应答 ,分别有 5 0 %和 72 %的患者可产生抗 - HBs,但与正常对照组 (86 % )相比明显低下 (P<0 .0 1) ;2 r HBc Ag可以诱导慢性 HBV携带者 PBMC产生抗 - HBc,同时亦可诱导抗 -HBe的产生 .结论 抗体应答水平低下可能是慢性乙型肝炎病毒携带者不能清除病毒的原因之一 .
Objective To investigate the antigen-induced antibody response in peripheral blood mononuclear cells (PBMCs) of chronic hepatitis B virus (HBV) carriers in vitro.Methods 18 patients with asymptomatic HBV carriers (ASC) and 18 patients with chronic hepatitis B (SC) .At the same time, 15 volunteers were selected as normal control, PBMCs were isolated and stimulated with HBsAg and r HBc Ag in vitro to detect the production of specific antibodies.Results 1 Asymptomatic HBV carriers and patients with chronic hepatitis B had varying degrees of antibody response, with anti-HBs produced in 50% and 72% of patients, respectively, but significantly lower than those in normal controls (86%) P <0.01). 2 r HBcAg can induce the production of anti-HBc in PBMC of chronic HBV carriers and also induce the production of anti-HBe.Conclusion The low level of antibody response may be due to chronic hepatitis B virus carriers can not clear One of the reasons for the virus.