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目的观察胃癌组织ｐ５３基因的超表达及其与预后的关系。方法用抗人Ｐ５３基因蛋白单克隆抗体Ｓ＿Ｐ免疫组织化学方法，观察１２８例胃癌组织ｐ５３表达状况，并对ｐ５３表达与胃癌淋巴结转移状态和术后５年生存率进行比较分析。结果胃癌组织１２８例的ｐ５３表达阳性率为４３８％（５６／１２８）；ｐ５３表达阳性和阴性组的胃癌局部和远处淋巴结转移率分别为６７９％（３８／５６）和５１４％（３７／７２），两者经统计学处理无显著性差异（Ｐ＞００５）。获得随访９８例，胃癌术后５年生存率的随访结果显示，ｐ５３阳性和阴性组分别为３８１％（１６／４２）和３０１％（１７／５６），两组间无统计学意义（Ｐ＞００５）。结论胃癌的发生与ｐ５３基因突变关系密切，并可用免疫组化检测，但Ｐ５３基因蛋白在胃癌组织中的超表达，似不能作为判断胃癌预后的参考指标，应进一步探讨
Objective To observe the overexpression of p53 gene in gastric cancer and its relationship with prognosis. Methods The p53 expression in 128 gastric cancer tissues was observed by immunohistochemical staining with anti-human P53 gene monoclonal antibody S_P. The p53 expression was compared with lymph node metastasis status and postoperative 5-year survival rate. Results The positive rate of p53 expression in gastric cancer tissues was 43.8% (56/128) in 128 cases; the local and distant lymph node metastasis rates in p53 positive and negative groups were 67.9% (38/56) and 51. 4% (37/72), there was no statistically significant difference between the two (P>005). Follow-up results were obtained in 98 cases. The 5-year survival rate of postoperative gastric cancer patients was 38.1% (16/42) and 30.1% (17/56) in the p53 positive and negative groups, respectively. There was no statistical difference between the two groups. Meaning (P>005). Conclusion The occurrence of gastric cancer is closely related to p53 gene mutation and can be detected by immunohistochemistry. However, the overexpression of P53 protein in gastric cancer may not be used as a reference index for judging the prognosis of gastric cancer. It should be further explored.