为了研究继代次数对暗褐网柄牛肝菌(Phlebopus portentosus)菌种活性的影响,选取生产上优良性状发生退化的栽培菌株11023为研究对象,监测其及继代2次,5次,8次后菌种的各项生理生化特征以及出菇性状。结果显示,随着继代次数增加,菌丝长势减弱,菌落生长速率降低,菌落颜色变浅,菌落边缘整齐度降低;淀粉酶分泌能力减弱;出菇率和单菇重均减小,抗杂能力减弱。推断菌株11023继代次数不宜超过2代。 In order to study the effect of subcultures on the activity of Phlebopus portentosus, the cultivated strain 11023 with excellent characters was selected as the research object, Subsequent species of the various physiological and biochemical characteristics and fruiting properties. The results showed that with the increasing of the number of subcultures, the mycelium growth was weakened, the colony growth rate was reduced, the colony color was shallow, the colony edge colony was reduced, the amylase secretion capacity was weakened, the fruiting rate and single mushroom weight were reduced, anti-miscellaneous Weakened ability. Inferred that strain 11023 subclass number should not exceed 2 generations.