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作者回顾了目前几种测定方法。WHO提倡的7天体内测定法,不能区分S和RI抗性;延长的28天试验,不易取得病人的密切合作,且其结果亦易被再感染混淆;体外大量法,需要静脉采血10ml,显然不适用于儿童;于是1978年Rieckmann等在大量法的基础上,根据Trager和Jensen的恶性疟原虫体外连续培养方法,用平底组织培养板(8×12井),设计出一种新的体外测定法——体外微量法。体外微量法将指尖或耳垂血100μl,与0.9ml培养液混匀,测试时用这种混合液加到合药的测定板的各井,每井50μl。并同时设有无药空白对照井。然后置于蜡烛缸中,37.5℃,孵育24～26小时。观察结果时,取各井红细胞沉淀物作厚片,根据各井内裂殖
The authors reviewed several current assays. The 7-day in vivo assay, advocated by the WHO, does not distinguish between S and RI resistance; an extended 28 day trial does not allow for close patient cooperation and the results are easily confounded by re-infection; in vitro large volumes require 10 ml venous blood sampling Not suitable for children; so in 1978 Rieckmann, etc., based on a large number of law, according to Trager and Jensen’s continuous culture of P. falciparum in vitro using flat tissue culture plate (8 × 12 wells), designed a new in vitro assay Method - in vitro micro-method. In vitro micro-fingertip or earlobe blood will be 100μl, and 0.9ml broth mix, test with this mixture was added to the assay plate wells, 50μl per well. At the same time there is no drug blank control wells. Then placed in a candle jar, 37.5 ℃, incubated for 24 to 26 hours. Observation results, take each well of erythrocyte sediment as a thick piece, according to the well in the fission