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GC-MS联用分析诺乙龙阳性尿,检出了诺乙龙经人体吸收代谢以后产生的九个代谢产物。除Met-2和Met-7在服药后84h的尿样中仍能被检出以外,诺乙龙原型及其它代谢物的体内存留时间较短,可检测时间不超过35h。通过解析质谱,鉴定了七个代谢物的分子结构,发现A环双键还原和D环羟基化是诺乙龙的两种体内代谢途径.用大孔树脂吸附提取、酶解、衍生化等处理尿样,方法回收率达80%,诺乙龙的检测下限为10ng/ml(10 ppb).
GC-MS was used for the analysis of Norprodil-positive urine. Nine metabolites produced after the absorption and metabolism of norobronone were detected. Except Met-2 and Met-7 can still be detected in the urine sample 84h after taking the drug, the body time of the prototype and other metabolites of Norprodron is shorter and the detectable time does not exceed 35h. Through analysis of mass spectrometry, the molecular structure of seven metabolites was identified. It was found that the double ring reduction of A ring and the hydroxylation of D ring are two kinds of in vivo metabolic pathways of norobronone. Adsorption extraction, enzymolysis, and derivatization of macroporous resin were used. For urine samples, the recovery rate of the method reached 80%, and the detection limit of norobronone was 10 ng/ml (10 ppb).