论文部分内容阅读
作者用免疫组织化学方法对40例8～38周人胎儿胃肠道5-HT免疫反应细胞(EC细胞)的发生进行了研究。收集水囊引产胎儿后,立即按胃体、胃窦、十二指肠、空肠、回肠及结肠6个部位取材,用Bouin液于4℃固定24小时,常规脱水,二甲苯透明,石蜡包埋,切片5μm。按Larsson的PAP法,对胃肠道中EC细胞进行定位观察(所用第一抗体为兔抗5-HT血清),用PBS代替兔抗5-HT血清作为对照。结果表明,该细胞最早见于8～9周胎儿十二指肠;12周以后,则从胃至结肠均可见到。十二指肠及回肠粘膜中EC细胞最多,
The authors used immunohistochemical methods 40 cases of 8 to 38 weeks of human fetal gastrointestinal tract 5-HT immune response cells (EC cells) were studied. Immediately after the fetus was collected, the fetuses were collected from the stomach, stomach antrum, duodenum, jejunum, ileum and colon. Bouin solution was fixed at 4 ° C for 24 hours. Conventional dehydration, xylene transparence and paraffin embedding , Sliced 5μm. According to Larsson’s PAP method, the EC cells in the gastrointestinal tract were located and observed (the primary antibody used was rabbit anti-5-HT serum), and PBS instead of rabbit anti-5-HT serum was used as a control. The results showed that the earliest seen in 8 to 9 weeks fetal duodenum; after 12 weeks, then from the stomach to the colon can be seen. Duodenal and ileal mucosa in the most EC cells,