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本文采用游离卵巢灌流的方法研究是否前列腺素(PG)合成受到抑制,然后抑制由促卵泡成熟激素(FSH)引起的排卵。研究用未成熟Spra gu(?)-Dawley大鼠,于生后28天晨注射20U孕马血(?)促性腺激素(PMSG),经此处理30天后引起卵泡成熟和内源性黄体生成素(LH)/FSH峰,在31人的早晨排卵。游离卵巢的外科技术及灌流介质的制备:动物麻醉后在腹主动脉进行插管,游离包括有右侧卵巢的一段血管,结扎所有与子宫和输卵管吻合的血管,并小心切开剥离卵泡囊暴露卵泡。将此卵巢制备标本放在灌流装置内。卵巢在装置内停止本身的流出液,在37℃含有80ml灌流液闭锁再循环的系统中进行灌流。灌流液为M199其中含有青霉素G钾75μg/ml,链霉素50μg/ml,硫酸盐肝素0.2μg/ml,胰岛素0.02IU/ml,和4%以游离脂肪酸为主的牛血清白蛋白。灌流液气化为含有95%
In this study, free ovarian perfusion was used to study whether prostaglandin (PG) synthesis was inhibited and then inhibited ovulation induced by follicle stimulating hormone (FSH). In immature Spra gu ~ (-) - Dawley rats, 20 U of pregnant mare’s blood (PMSG) was injected 28 days after birth to induce follicular maturation and endogenous luteinizing hormone (LH) / FSH peak ovulation in 31 people in the morning. Surgical technique of free ovary and preparation of perfusion medium: The animal is intubated in the abdominal aorta after anesthesia, a section of blood vessel including the right ovary is free, blood vessels that match the uterus and fallopian tube are ligated, and the follicular capsule Follicles. This ovarian preparation was placed in a perfusion device. The ovaries stop their own effluent within the device and are perfused in a system containing a closed loop of 80 ml of perfusate at 37 ° C. The perfusate was M199 containing 75 μg / ml penicillin G potassium, 50 μg / ml streptomycin, 0.2 μg / ml sulphate heparin, 0.02 IU / ml insulin, and 4% free fatty acid based bovine serum albumin. Perfusion fluid gasification to contain 95%