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目的研究胶原蛋白海绵生产中病毒灭活工艺及其灭活病毒的效果。方法采用细胞培养法,对酸性酶解溶液和60Co-γ射线辐照法灭活病毒的效果进行了检测。结果酸性酶解溶液处理2 h,对接种在胶原蛋白海绵中的水疱性口炎病毒、伪狂犬病毒、脊髓灰质炎病毒和猪细小病毒灭活对数值分别为5.54、5.71、6.30和5.13。用照射剂量为25 kGy的60Co-γ射线对胶原蛋白冻干品中的水疱性口炎病毒、伪狂犬病毒、脊髓灰质炎病毒和猪细小病毒灭活对数值分别为6.00、5.75、6.00和5.00。灭活后的样品经细胞感染盲传3代均未见细胞病变。结论胶原蛋白海绵生产中采用酸性酶解溶液处理72 h或25kGy60Co-γ射线辐照灭菌工艺均可有效灭活所试验的4种指示病毒。
Objective To study the virus inactivation process of collagen sponge and the effect of virus inactivation. Methods The cell culture method was used to detect the effect of inactivation of virus by acid hydrolysis solution and 60Co-γ ray irradiation. Results The inactivation logarithm of vesicular stomatitis virus, pseudorabies virus, poliovirus and porcine parvovirus vaccinated in collagen sponge were 5.54, 5.71, 6.30 and 5.13 respectively after being treated with acidic enzymolysis solution for 2 h. Inactivation of vesicular stomatitis virus, pseudorabies virus, poliovirus and porcine parvovirus in collagen lyophilates were 60.00%, 60.00%, 5.00% and 60.00%, respectively, with 60Co-γ rays irradiated at a dose of 25 kGy . After inactivation of samples blind infection by cell infection 3 generations have not seen cytopathic effect. CONCLUSIONS: The four indicator viruses tested were effectively inactivated by irradiation with acid enzymolysis solution for 72 h or irradiation with 25 kGy60Co-γ-ray.