论文部分内容阅读
目的：探讨星形胶质细胞瘤的恶性程度与细胞凋亡、细胞增殖的相关性。方法：利用 Ｄ Ｎ Ａ 凝胶电泳确定３４ 例肿瘤细胞群体中细胞凋亡的存在，使用体视学方法计数凋亡指数（ Ａ Ｉ） 、增殖指数（ Ｍ Ｉ） 、 Ａ Ｉ／ Ｍ Ｉ比率。结果： Ｄ Ｎ Ａ 凝胶电泳２６ 例显示特征性的 Ｄ Ｎ Ａ 梯带， Ａ Ｉ、 Ｍ Ｉ分别随肿瘤恶性程度增加而递增，且 Ａ Ｉ与 Ｍ Ｉ呈正相关，但 Ａ Ｉ／ Ｍ Ｉ随恶性度增高而降低。结论：肿瘤细胞群体中存在细胞凋亡，恶性肿瘤中细胞的增殖远活跃于细胞凋亡。 Ａ Ｉ／ Ｍ Ｉ比率提示了细胞凋亡与增殖的关系。
Objective: To investigate the relationship between the degree of malignancy of astrocytoma and apoptosis and cell proliferation. METHODS: The presence of apoptosis in 34 tumor cell populations was determined by D N A gel electrophoresis. The apoptotic index (A I), proliferation index (M I), and A I/M I ratio were counted using stereological methods. Results: D N A gel electrophoresis in 26 cases showed characteristic D N A ladders, and A I and M I increased with the increase of tumor malignancy, and A I was positively correlated with M I, but A I/M I was associated with Malignancy increased and decreased. Conclusion: There is apoptosis in the tumor cell population. The proliferation of cells in malignant tumors is far more active than apoptosis. The A/M I ratio suggests a relationship between apoptosis and proliferation.