本文选用健康SD大鼠160只,用PH+DENA+PB诱癌模型,即:在肝大部切除术(PH)后18-24hrs腹腔注射二乙基亚硝胺(DENA)10mg/kg进行启动,一周后饮水中加入0.05％的苯巴比妥(PB)进行促进,在1周和1、2、3、4、5、7和10月末分批股动脉放血处死动物,用H.E.染色、酶组化和免疫组化对照观察和体视学定量的方法,对该模型产生的肝癌中间病变的演变过程和转变灶、结节发生的时间、部位、性别差异进行了研究。 In this study, 160 healthy SD rats were selected and the PH+DENA+PB carcinogenesis model was used, that is, 18-24 hrs of intraperitoneal injection of diethylnitrosamine (DENA) 10 mg/kg was initiated after subtotal hepatectomy (PH). One week later, drinking water was added with 0.05% phenobarbital (PB) to promote, and animals were sacrificed by femoral artery bleeding at the end of 1 week and 1, 2, 3, 4, 5, 7 and 10 months, and stained with HE and enzyme. The histological and immunohistochemical methods of control observation and stereological quantification were used to investigate the evolution process of hepatocellular carcinoma intermediate lesions produced by this model and the time, location, and sex differences of the lesions and nodules.