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为了检测角膜有无ＨＢＶ－ＤＮＡ，防止角膜移植传播乙型肝炎病毒（ＨＢＶ），应用ＰＣＲ技术对２９例乙型肝炎、乙型肝炎病毒感染患者和１０例健康人角膜的ＨＢＶ－ＤＮＡ进行了检测。同时对１１例乙型肝炎病毒感染患者的结膜和２６例乙型肝炎患者的泪液也进行了检测。结果ＨＢｓＡｇ阳性的１６例角膜有１１例（６８．７％）检测到ＨＢＶ－ＤＮＡ，１３例ＨＢｓＡｇ阴性的角膜有４只（３６．６％）ＨＢＶ－ＤＮＡ阳性，１０例健康人角膜无１例ＨＢＶ－ＤＮＡ阳性。１１例乙型肝炎病毒感染患者的结膜中，有７例（６３．６％）ＨＢＶ－ＤＮＡ阳性，２６例乙型肝炎患者的泪液中，有１４例（５３．８％）检测到ＨＢＶ－ＤＮＡ。说明角膜也是ＨＢＶ的贮存场地，临床不能应用乙型肝炎病毒感染者的角膜作供体行角膜移植术
In order to detect the existence of corneal HBV-DNA and prevent the transmission of hepatitis B virus (HBV) by corneal transplantation, the HBV-DNA of 29 patients with hepatitis B, hepatitis B virus infection and 10 healthy human cornea were detected by PCR . At the same time, 11 cases of hepatitis B virus infection in patients with conjunctiva and 26 cases of hepatitis B patients were also tested for tears. Results HBV-DNA was detected in 11 of 16 (68.7%) HBsAg-positive corneas, 4 (36.6%) of 13 HBsAg negative corneas, and 1 in 10 healthy human corneas HBV-DNA positive. Of the 11 patients with hepatitis B virus infection, 7 (63.6%) had positive HBV DNA in the conjunctiva and 14 (53.8%) of 26 patients with hepatitis B had HBV DNA . Corneal also shows that the cornea is also a storage site for clinical use of hepatitis B virus can not be applied to corneal donor corneal transplant