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目的探索改进阴道毛滴虫封存标本的制作方法。方法取附属医院妇科门诊阴道毛滴虫患者的阴道分泌物,在无菌条件下接种于改良的肝浸汤培养基中,加入2ml无菌健康马血清及5×104U/ml青霉素,置37℃培养箱内培养,选取转种3代培养36h的阴道毛滴虫,采用载玻片的包装隔离纸带拖片,经改进的染液染色制片。结果封片标本中虫体分布均匀,伸展好。细胞浆呈淡蓝色,波动膜、轴柱、鞭毛呈蓝色,核红色,核仁紫红色。结论采用本方法制作的阴道毛滴虫封片标本虫体结构清晰,形态特征明显。
Objective To explore ways to improve the storage of specimens of Trichomonas vaginalis. Methods Vaginal secretions of Trichomonas vaginalis patients in gynecology clinic of Affiliated Hospital were inoculated into modified liver immersion culture medium under sterile conditions. 2 ml of sterile healthy horse serum and 5 × 104 U / ml penicillin were added and placed at 37 ℃ Incubation in the incubator, selected three generations of transplanted 36h vaginal Trichomonas vaginalis, the use of glass slide packaging isolation tape mosaics, the improved dye staining film. Results The specimens were evenly distributed and stretched well. Cytoplasm was light blue, fluctuating membrane, shaft column, flagella were blue, nuclear red, purple nucleolus. Conclusion The method of producing Trichomonas vaginalis specimen specimens clear body structure, the obvious morphological characteristics.