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以甘草幼苗为实验材料,研究了外源蔗糖对NaCl胁迫条件下甘草幼苗生长及有效成分含量的影响。结果表明,在盐胁迫条件下,添加一定浓度的外源蔗糖后,甘草幼苗的日相对生长率呈升高趋势,可明显的消弱这种胁迫效应,说明外源蔗糖具有缓解盐胁迫的效应。总黄酮含量和苯丙氨酸解氨酶(PAL)活性呈极显著升高,外源蔗糖对盐胁迫下甘草幼苗根系生长的缓解作用在很大程度上与PAL活性的增强所引起的黄酮含量的增加有关,当外源蔗糖的浓度为10 mmol·L~(-1)时,PAL的活性达到最大,当外源蔗糖的浓度为15 mmol·L~(-1)时,PAL的活性又成下降趋势,表明这种缓解作用具有浓度效应。在施加不同浓度的外源蔗糖后甘草苷含量的变化跟总黄酮含量的变化相似,施加不同浓度的外源蔗糖后甘草酸含量比在盐胁迫下高,达到未受盐胁迫时的水平,外源蔗糖的浓度梯度对甘草酸积累的影响不明显。
The effects of exogenous sucrose on the growth of Glycyrrhiza uralensis seedlings under the stress of NaCl stress and the content of active ingredients were studied using licorice seedlings as experimental material. The results showed that under the condition of salt stress, the relative growth rate of Glycyrrhiza uralensis seedlings increased with the addition of a certain concentration of exogenous sucrose, which could obviously weaken the stress effect, indicating that exogenous sucrose has the effect of alleviating salt stress . The content of total flavonoids and the activity of phenylalanine ammonia lyase (PAL) increased extremely significantly. The exogenous sucrose alleviated the root growth of licorice seedlings under salt stress to a great extent with the increase of PAL activity The activity of PAL reached the maximum when the concentration of exogenous sucrose was 10 mmol·L -1. When the concentration of exogenous sucrose was 15 mmol·L -1, the activity of PAL A downward trend, indicating that the mitigation effect with concentration. After applying different concentrations of exogenous sucrose, the content of glycyrrhizin changed with the change of total flavonoids. After applying different concentrations of exogenous sucrose, the content of glycyrrhizin was higher than that under salt stress and reached the level without salt stress. The concentration gradient of source sucrose had no obvious effect on glycyrrhizin accumulation.