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WRKY转录因子家族在植物抵御各种生物、非生物胁迫反应和生长发育调控中扮演着重要角色。利用生物信息学方法鉴定和分析棉花A基因组WRKY转录因子,共鉴定得到109个候选WRKY基因,编码区全长474~3 528 bp,基因分布在棉花13条染色体上。依据WRKY家族基因的保守结构域和系统进化关系,将其划分为3个大组:G1组、G2组和G3组。G1组WRKY基因包括21个成员,其中18个含有两个WRKY域;G2组有73个成员,可进一步分为G2-a、G2-b、G2-c、G2-d和G2-e五个亚类;G3组WRKY基因包括15个成员。同组的WRKY成员保守域的氨基酸构成大体相似,但也存在WRKYGQK多肽和锌指结构的变异,不同组成员的氨基酸保守域组成特异。拟南芥和棉花WRKY基因的系统进化树表明,功能相似的基因聚在一起,暗示和拟南芥抗逆功能基因聚类在一起Ga WRKY可能参与棉花抵御逆境胁迫反应,为进一步发现和挖掘棉花WRKY基因的功能奠定理论基础。
The WRKY transcription factor family plays an important role in plant defense against a variety of biological, abiotic stress responses and growth and development regulation. The WRKY transcription factor of cotton A genome was identified and analyzed by bioinformatics method. A total of 109 candidate WRKY genes were identified. The coding region of the WRKY gene was 474-3 528 bp in length, and the gene was located on 13 chromosomes of cotton. According to the conserved domains of WRKY family genes and phylogenetic relationships, we divided them into three groups: G1, G2 and G3. The G1 WRKY gene consists of 21 members, of which 18 contain two WRKY domains; the G2 group has 73 members which can be further divided into five G2-a, G2-b, G2-c, G2-d and G2- Subgroup; G3 group WRKY gene includes 15 members. Amino acid composition of conserved domains of WRKY members in the same group are similar, but there are also variations of WRKYGQK polypeptide and zinc finger structure. The amino acid conserved domains of different groups of members are composed specifically. The phylogenetic tree of WRKY genes from Arabidopsis and cotton indicated that the clustering of genes with similar functions suggested that Arabidopsis thaliana resistant genes clustered together. Ga WRKY may be involved in the cotton response to stress. In order to further discover and mine cotton WRKY gene function laid the theoretical basis.