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目的：观察大鼠血清中细胞因子在急性胰腺炎发病机理中的作用。方法：检测血清中细胞因子和TNF-α，观察胰、肝、肺、肾的病理变化及胰腺细胞培养液中淀粉酶和PLA_2化。结果：在坏死性胰腺炎和水肿性胰腺炎大鼠血清中，IL-1β、IL-6、IL-2和TNF-α都明显高于正常对照组(P＜0.01），水肿性和坏死性胰腺炎的胰组织中4种细胞因子都高于正常胰腺组（P＜0.01），但两者之间未见明显差别。水肿性胰腺炎分离培养的胰腺细胞上清液中测出IL-6和TNF-α都高于正常胰腺组。结论:急性胰腺炎症过程中有多种细胞因子参与发病机理，其在体内放大作用造成胰腺本身在内的全身多器官功能损害，而且细胞因子的升高与疾病的严重程度相关。
Objective: To observe the effect of serum cytokines in the pathogenesis of acute pancreatitis in rats. Methods: Serum cytokines and TNF-α were detected. Pathological changes in pancreas, liver, lung and kidney were observed, and amylase and PLA2 in pancreatic cell culture medium were detected. Results: The levels of IL-1β, IL-6, IL-2 and TNF-α in serum of necrotizing pancreatitis and edematous pancreatitis rats were significantly higher than those of normal control group (P <0.01), edema and necrosis Pancreatitis pancreatic tissue in the four cytokines were higher than the normal pancreas group (P <0.01), but no significant difference between the two. The pancreatic cell supernatant isolated from edematous pancreatitis was found to have higher IL-6 and TNF-α levels than the normal pancreas group. CONCLUSIONS: A variety of cytokines are involved in the pathogenesis during the acute pancreatitis. They amplify the systemic multiple organ dysfunction caused by the enlargement of the pancreas in vivo, and the increase of cytokines is related to the severity of the disease.