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目的:研究两种来源不同的食管鳞癌细胞系EC109和KYSE510分裂增殖能力和侵袭能力的差异。方法:利用MTT实验和侵袭实验,在体外分别检测EC109和KYSE510细胞的分裂增殖能力和侵袭能力;采用裸鼠皮下接种实验和爪垫皮下接种淋巴结转移模型,比较两种细胞在体内的成瘤能力、局部侵袭能力和区域淋巴结转移能力的差异;免疫印迹检测上皮-间充质细胞转换标志蛋白E-cadherin、γ-cadherin和Vimentin在两种细胞中的表达水平。结果:体外实验表明,EC109细胞的分裂增殖和侵袭能力均明显较KYSE510细胞的强(P<0.05);皮下接种和爪垫皮下接种淋巴结转移实验显示EC109细胞的成瘤能力、局部侵袭能力和淋巴结转移能力均较KYSE510细胞的高;免疫印迹检测发现,E-cadherin和γ-cadherin在EC109细胞中的表达水平较KYSE510细胞中的表达低,而Vimentin在EC109细胞中的表达则较KYSE510细胞中的表达高,提示EC109细胞发生上皮-间充质细胞转换的程度较KYSE510的高。结论:EC109细胞的增殖和侵袭能力均较KYSE510细胞的强,上皮-间充质细胞转换可能是导致这种差异的原因之一。
OBJECTIVE: To study the differences in the proliferation and invasion of EC109 and KYSE510 cells from two different esophageal squamous cell carcinoma cell lines. Methods: MTT assay and invasion assay were used to detect the proliferation and invasion ability of EC109 and KYSE510 cells in vitro. The nude mice were inoculated subcutaneously with nude mice and lymph node metastasis model , Local invasion ability and regional lymph node metastasis ability. Western blotting was used to detect the expression of E-cadherin, γ-cadherin and Vimentin in epithelial-mesenchymal cells. Results: In vitro experiments showed that the proliferation and invasion ability of EC109 cells were significantly stronger than that of KYSE510 cells (P <0.05). The subcutaneous inoculation and subcutaneous inoculation lymph node metastasis of EC109 cells showed the ability of tumorigenesis, local invasion and lymph node metastasis Western blot analysis showed that the expression of E-cadherin and γ-cadherin in EC109 cells was lower than that in KYSE510 cells, while the expression of Vimentin in EC109 cells was higher than that in KYSE510 cells High expression, suggesting EC109 cells epithelial-mesenchymal transition than KYSE510 high. CONCLUSION: The stronger epithelial-mesenchymal transition of EC109 cells than KYSE510 cells may be one of the reasons for this difference.