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目的:优选柘木中总黄酮的提取工艺,为柘木总黄酮的开发利用提供参考。方法:采用L9(34)正交试验,以柘木总黄酮的含量和出膏率为综合指标,考察乙醇浓度、乙醇用量、提取时间和提取次数4个因素对提取效果的影响。结果:最佳提取工艺为12倍量60%乙醇提取3次,1.5h/次。结论:该工艺合理,稳定可行,可为柘木总黄酮提取工艺的确定提供实验依据。
Objective: To optimize the extraction technology of total flavonoids from Alnus cremastogyne, provide a reference for the development and utilization of aloe flavone. Methods: L9 (34) orthogonal test was used to study the effect of total flavonoids content and the rate of ointment on the extraction efficiency. The effects of ethanol concentration, ethanol dosage, extraction time and extraction times were investigated. Results: The best extraction process was 12 times the amount of 60% ethanol extraction 3 times, 1.5h / time. Conclusion: The process is reasonable, stable and feasible, which can provide experimental evidence for the determination of total flavonoids extraction process.