论文部分内容阅读
目的建立电感耦合等离子体质谱法(ICP-MS)测定大鼠血清及各组织中铅和钙含量,探讨大鼠经肺染纳米硫化铅后其体内钙含量的变化。方法选择清洁级雄性大鼠随机分为高剂量组、低剂量组和对照组,染毒后经心脏取血,并取心脏、肝脏、肺、肾脏、股骨、大脑皮质和海马组织,经消化后,使用内标法对铅、钙基体干扰进行校正,采用ICP-MS法测定大鼠血清及各组织中的铅、钙含量。结果随着铅加入量的增加,除股骨外,大鼠血清和各组织中钙含量逐渐下降,对照组明显高于高剂量组(P<0.05);而大鼠血清及各组织中铅含量逐渐升高,对照组明显低于高剂量组(P<0.05),尤其是低、高剂量组股骨中铅含量增加量明显,说明股骨是铅的主要蓄积器官;该方法测定钙的精密度为1.08%~3.41%,回收率平均值为102.1%;测定铅的精密度为1.67%~3.10%,回收率平均值为105.8%。结论该方法适合于样品量小且元素含量差异大的生物样品;纳米硫化铅在大鼠体内的蓄积可能会影响钙元素的代谢。
Objective To establish a method for the determination of lead and calcium in serum and tissues of rats by inductively coupled plasma mass spectrometry (ICP-MS), and to explore the change of calcium content in rat after lung-stained with lead sulfate. Methods Clean male rats were randomly divided into high dose group, low dose group and control group. The hearts were taken from the heart after exposure to the virus and the heart, liver, lung, kidney, femur, cerebral cortex and hippocampus were harvested. After digestion The internal standard method was used to correct the interference of lead and calcium matrix. The contents of lead and calcium in serum and tissues of rats were determined by ICP-MS. Results With the addition of lead, the content of calcium in serum and tissues of rats decreased gradually except for the femur, which was significantly higher in the control group than in the high-dose group (P <0.05); while the content of lead in the serum and tissues of rats gradually (P <0.05). Especially in the low and high dose groups, the increase of lead content in the femur was significant, indicating that the femur was the main accumulation organ of lead. The precision of determination of calcium in this method was 1.08 % ~ 3.41%, the average recovery was 102.1%; the precision of determination of lead was 1.67% ~ 3.10%, the average recovery was 105.8%. Conclusion The method is suitable for biological samples with small sample amount and large difference in elemental content. The accumulation of lead sulfide in rats may affect the metabolism of calcium.