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Objective:To explore the regulatory mechanism of Xiaoyin Recipe(消银方) on the T helper 1/ T helper 2(Th1/Th2) immune balance.Methods:Thirty-six experimental animals were divided into three groups, 12 rats in each group:blank control group(B group),negative control group(N group),and Xiaoyin Recipe treatment group(T group).The latter two groups received immunization of experimental autoimmune thyroiditis (EAT),and T group were treated with Xiaoyin Recipe for a month.Then,the expression of Th1-Th2-related genes in peripheral blood mononuclear cells(PBMCs) were screened with Oligo GEArray Rat Th1-Th2-Th3 Microarray.The expressions of tumor necrosis factor-α(TNF-α),interleukin-10(IL-10),T-box expressed in T-cells(T-bet),and GATA-binding protein-3(GATA-3) were detected by real-time polymerase chain reaction (RT-PCR).Results:Gene array screening showed that compared to N group,in T group after Xiaoyin Recipe treatment,3 genes were upregulated in EAT rats,including interleukin-27 receptor alpha(IL-27rα),glomulin (Glmn),and GATA-3,while 38 genes were downregulated,such as CD28,IL-18,signal transducer,and activator of transcription 1(STAT1),T-bet,TNF receptor superfamily member 4(TNFRSF4),TNF ligand superfamily member 5(TNFSF5),and TNF receptor superfamily member 5(TNFRSF5).While RT-PCR showed that there was an increased level of TNF-αmRNA(P<0.01),an elevated ratio of T-bet/GATA-3,and a decreased level of IL-10 mRNA in PBMC of N and T group compared to B group(P<0.01);and after treatment with Xiaoyin Recipe, IL-10 mRNA level increased(P<0.01),while TNF-a mRNA level and T-bet/GATA-3 ratio decreased in T group compared to N group(P<0.01).Conclusion:Xiaoyin Recipe for psoriasis could induce a Th1/Th2 balance drift toward Th2 in PBMC of EAT rats and thus improve the conditions.
Objective: To explore the regulatory mechanism of Xiaoyin Recipe on the T helper 1 / T helper 2 (Th1 / Th2) immune balance. Methods: Thirty-six experimental animals were divided into three groups, 12 rats in each group : blank control group (B group), negative control group (N group), and Xiaoyin Recipe treatment group (T group). latter latter groups immunization of experimental autoimmune thyroiditis (EAT), and T group were treated with Xiaoyin Recipe for a month. Chen, the expression of Th1-Th2-related genes in peripheral blood mononuclear cells (PBMCs) were screened with Oligo GE Array Rat Th1-Th2-Th3 Microarray. These expressions of tumor necrosis factor-a (TNF- T-box expressed in T-cells (T-bet), and GATA-binding protein-3 (GATA-3) were detected by real-time polymerase chain reaction : Gene array screening showed that compared to N group, in T group after Xiaoyin Recipe treatment, 3 genes were upregulated in EAT rats, including interleukin-27 recept while 38 genes were downregulated, such as CD28, IL-18, signal transducer, and activator of transcription 1 (STAT1), T-bet, TNF receptor (TNFRSF4), TNF ligand superfamily member 5 (TNFSF5), and TNF receptor superfamily member 5 (TNFRSF5) .While RT-PCR showed that there was an increased level of TNF-a mRNA (P <0.01) of T-bet / GATA-3, and a decreased level of IL-10 mRNA in PBMC of N and T group compared to B group (P <0.01); and after treatment with Xiaoyin Recipe <0.01) while TNF-α mRNA level and T-bet / GATA-3 ratio decreased in T group compared to N group (P <0.01) .Conclusion: Xiaoyin Recipe for psoriasis could induce a Th1 / Th2 balance drift toward Th2 in PBMC of EAT rats and thus improve the conditions.