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目的:探讨羊栖菜多糖(SFPS)对软脂酸诱导的肝细胞凋亡的保护作用及其可能机制。方法:SFPS不同浓度(25、50、100μg/mL)预处理24h后,0.5mmol/L软脂酸(PA)作用48h诱导正常人肝细胞株(HL-7702)凋亡。MTT比色法检测SFPS对细胞增殖的影响;Annexin V-FITC标记法检测细胞的凋亡;RT-PCR法检测凋亡调控基因Bcl-2、Bax的表达并计算其比值(Bcl-2/Bax)。结果:0.5mmol/L PA处理48h的HL-7702细胞存活率下降为正常对照组的64.9%,细胞凋亡率为35.1%,Bax表达上升,Bcl-2表达及Bcl-2/Bax比值明显下降(均P<0.05);与模型组相比,SFPS不同浓度(25、50、100 ug/ml)预处理后细胞存活率逐渐升高,细胞凋亡率显著降低,Bax表达逐渐下降,Bcl-2表达及Bcl-2/Bax比值逐渐上升(均P<0.05)。结论:SFPS对PA诱导的肝细胞凋亡有较好的保护作用,其作用机制可能与调节Bcl-2、Bax基因的表达有关。
Objective: To investigate the protective effect of Sargassum polysaccharide (SFPS) on palmitate-induced hepatocyte apoptosis and its possible mechanism. Methods: Apoptosis of normal human hepatocyte cell line HL-7702 was induced by 0.5 mmol / L palmitate (PA) for 48 h after pretreatment with SFPS at different concentrations (25, 50 and 100 μg / mL) MTT assay was used to detect the effect of SFPS on cell proliferation; Annexin V-FITC method was used to detect cell apoptosis; Bcl-2 / Bax expression was detected by RT-PCR; ). Results: The survival rate of HL-7702 cells treated with 0.5mmol / L PA for 48h decreased to 64.9% of the normal control group, the apoptosis rate was 35.1%, the expression of Bax increased, the expression of Bcl-2 and the ratio of Bcl-2 / (P <0.05). Compared with the model group, the cell survival rate of SFPS pretreated with different concentrations (25,50,100 ug / ml) increased gradually, the apoptosis rate decreased significantly, the expression of Bax decreased gradually, the expression of Bcl- 2 expression and the ratio of Bcl-2 / Bax gradually increased (all P <0.05). CONCLUSIONS: SFPS has a good protective effect on hepatocellular apoptosis induced by PA, and its mechanism may be related to the regulation of Bcl-2 and Bax gene expression.