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采用ＥＬＩＳＡ法检测３２例ＮＳ患儿及２４例健康对照儿童血清和尿液ｓＩＬ－２Ｒ浓度并采用单克隆抗体间接免疫荧光法检测Ｔ细胞亚群。结果：①ＮＳ活动期组患儿血清及尿液ｓＩＬ－２Ｒ浓度明显高于ＮＳ缓解期组（Ｐ＜０．０１）和健康对照组（Ｐ＜０．０１），而ＮＳ缓解期组血清及尿液ｓＩＬ－２Ｒ浓度与健康对照组间无显著性差异（Ｐ＞０．０５）；②ＮＳ活动期患儿尿液ｓＩＬ－２Ｒ与血清ｓＩＬ－２Ｒ浓度呈正相关（Ｒ＝０．５３８６；Ｐ＜０．０５）；③ＮＳ活动期患儿ＣＤ＋４细胞数及ＣＤ＋４／ＣＤ＋３细胞比值明显低于ＮＳ缓解期和健康对照组（Ｐ＜０．０１）。表明ＮＳ活动期患儿Ｔ细胞免疫应答能力降低；ｓＩＬ－２Ｒ浓度升高可作为肾病综合征活动指标之一。
Serum and urine concentrations of sIL-2R were detected by ELISA in 32 children with NS and 24 healthy controls. T lymphocyte subsets were detected by indirect immunofluorescence with monoclonal antibody. Results: ① The levels of sIL-2R in serum and urine in NS active group were significantly higher than those in NS remission group (P <0.01) and healthy control group (P <0.01), while those in NS remission group were significantly higher than those in NS remission group There was no significant difference between sIL-2R and sIL-2R concentrations in urine (P> 0.05). ②The sIL-2R in urine of NS patients was positively correlated with serum sIL-2R .05). ③ The ratio of CD + 4 cells and CD + 4 / CD + 3 cells in NS active stage was significantly lower than that in NS remission stage and healthy control group (P <0.01). Indicating that children with NS activity of T-cell immune response to reduce the capacity; sIL-2R concentration can be used as an indicator of nephrotic syndrome activity.