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目的探讨脂多糖(LPS)对BV2细胞肿瘤坏死因子α(TNF-α)、簇分化抗原11b(CD11b)和白细胞介素1β(IL-1β)表达的影响。方法分别用LPS 0、1、10、100μg/ml和LPS 10μg/ml以0、2、4、8、12、24h刺激BV2细胞,ELISA法检测细胞培养上清液中分泌的TNF-α表达量,RT-PCR检测CD11b及IL-1βmRNA表达变化。结果用LPS 10μg/ml刺激BV2细胞4h后,上清液中TNF-α的释放量达到峰值,同时CD11b及IL-1βmRNA表达明显增加。结论 LPS能促进BV2细胞高效表达CD11b、IL-1β和分泌TNF-α,从而为BV2细胞作为免疫活性细胞在中枢神经系统中发挥免疫调节作用提供依据。
Objective To investigate the effect of lipopolysaccharide (LPS) on the expression of tumor necrosis factor α (TNF-α), cluster-derived antigen 11b (CD11b) and interleukin-1β (IL-1β) in BV2 cells. Methods BV2 cells were stimulated with LPS at 0, 1, 10, 100μg / ml and LPS 10μg / ml for 0, 2, 4, 8, 12 and 24 hours respectively. The secreted TNF- The expression of CD11b and IL-1βmRNA were detected by RT-PCR. Results After BV2 cells were stimulated with LPS 10μg / ml for 4h, the release of TNF-α in the supernatant peaked and the expressions of CD11b and IL-1β mRNA increased significantly. CONCLUSION: LPS can promote the expression of CD11b, IL-1β and TNF-α in BV2 cells, and provide a basis for BV2 cells to play an immunoregulatory role in central nervous system.