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【目的】利用Mutator(Mu)诱变群体获得、验证叶色白化突变体的Mu因子插入位点;解析玉米叶色变异相关基因及其代谢网络。【方法】以含有活性MuDR转座子的W22∷Mu为父本,与玉米自交系综31(Z31)杂交产生的M2和M3家系群体为材料,经表型性状的遗传分析和Mu插入位点的分离获得白化突变体,经生物信息学方法解析白化突变体产生的相关基因,同时构建产生白化突变代谢网络。【结果】对870个M2家系的16000余单株和M3种植的36个家系近700单株进行考察,获得了遗传稳定的白化突变体41株。经Mu-TAIL-PCR分析获得35条Mu因子插入序列;对靶位点序列分析,获得的14个靶位点可能与叶绿素合成及光合作用有关;利用其中6个靶位点构建了5条玉米叶色突变产生白化的叶绿素代谢网络途径。【结论】初步证实了Mu转座子插入的27个靶位点与叶色白化突变体的关联;建立了6个靶位点的玉米叶色突变产生白化的叶绿素代谢网络途径。
【Objective】 Mutant (Mu) mutants were used to confirm the insertion of Mu factor in leaf albino mutants. Maize leaf color-related genes and their metabolic networks were analyzed. 【Method】 The M2 and M3 pedigree populations derived from crossing W22 :: Mu with active MuDR transposon and maize inbred line Zu31 (Z31) were used as materials. Genetic analysis and phenotypic traits Point separation of albinism mutants, bioinformatics analysis of albinism mutants generated genes, while building albino mutant metabolic network. 【Result】 The results showed that more than 16,000 plants from 870 M2 families and nearly 700 plants from 36 families from M3 planting were investigated, and 41 genetically stable albino mutants were obtained. 35 Mu factor insertions were obtained by Mu-TAIL-PCR analysis. Sequence analysis of the target sites indicated that the 14 target sites may be related to chlorophyll synthesis and photosynthesis. Six of these five target sites were used to construct 5 maize Leaf color mutations produce a bleached chlorophyll metabolic network pathway. 【Conclusion】 The results showed that the 27 target loci inserted by Mu transposon were positively correlated with the leaf color albino mutants. A phytochemical pathway of chlorophyll metabolism in maize leaves with six target sites was established.