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实验建立了检测肝细胞膜低密度脂蛋白受体的抗配体抗体酶联免疫吸附测定法．测定中，固相膜受体蛋白量由包放前后膜蛋白浓度差值计算确定；低密度脂蛋白结含量按双抗体夹心法制作的低密度脂蛋白标准曲线确定，膜蛋白非村异吸附则用与酶联抗体来源相同的同种动物血浆低密度脂蛋白平行抑制试验消除．兔肝细胞膜低密度脂蛋白受体结合活性经Scatchard作图，Kd＝13．6mg／L，Bmax＝124μg／g膜蛋白（n=5）．测定结果说明建立的方法安全可靠，能反映受体的结合活性，在高脂血症及心血管病的研究中具有广泛的应用价值．本研究还对选择确定受体包被浓度、酶交联物稀释度等的棋盘滴定方法作了详细介绍和讨论．
An anti-Ligand antibody enzyme-linked immunosorbent assay (ELISA) was developed for the detection of low density lipoprotein receptor in liver cell membrane. Determination, the amount of solid-phase membrane protein receptor protein concentration package before and after the difference between the calculated value of the determination; low-density lipoprotein content according to double-antibody sandwich production of low-density lipoprotein standard curve to determine the non-membrane protein adsorption Using the same source of enzyme-linked antibody plasma low density lipoprotein parallel inhibition test to eliminate. Rabbit hepatocytes membrane LDL receptor binding activity by Scatchard plot, Kd = 13.6mg / L, Bmax = 124μg / g membrane protein (n = 5). The results of the assay indicate that the established method is safe and reliable and can reflect the binding activity of the receptor and has broad application value in the study of hyperlipidemia and cardiovascular diseases. This study also made a detailed introduction and discussion on the method of chessboard titration for determining the concentration of the receptor, the dilution of the enzyme cross-linker and the like.