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探讨幽门螺杆菌（ Ｈｐ）感染与胃癌发病的关系。收集４９例经胃镜诊断为胃溃疡或十二指肠溃疡患者的血清和２０例正常健康人血清，分别用放免法（ Ｒ Ｉ Ａ）测定血清中胃泌素水平， Ｅ Ｌ Ｉ Ｓ Ａ 法测定血清中抗 Ｈｐ Ｉｇ Ｇ、 Ｉｇ Ａ和 Ｉｇ Ｍ 水平，根据血清抗体检测的结果，分为４组观察患者血清对体外培养的人胃癌细胞 Ｈ Ａ Ｃ８０３、人结肠癌细胞 Ｈ Ｔ２９增殖的影响，细胞增殖试验采用 Ｍ Ｔ Ｔ 法测定。结果：胃及十二指肠溃疡患者血清胃泌素（１３９．６±３８１７ｐｇ／ｍ ｌ）明显高于正常人（６３．１２±２８．７ｐｇ／ｍ ｌ），两者差异有显著性（ Ｐ＜ ０．０１）。胃及十二指肠溃疡患者血清中，高 Ｈｐ Ｉｇ Ｇ 抗体滴度组（ Ａ 组）和高 Ｈｐ Ｉｇ Ａ 抗体滴度组（ Ｂ组）对人胃癌细胞具有明显的促增殖作用 （ Ｐ＜ ００５），尤其是高 Ｈｐ Ｉｇ Ａ 抗体滴度 Ｂ组的作用更为明显。而４组患者血清对人结肠癌细胞都没有明显的促增殖作用（ Ｐ＞ ００５）。结论：受 Ｈｐ 感染的胃及十二指肠溃疡患者血清中，高滴度的 Ｈｐ Ｉｇ Ｇ 组和 Ｈｐ Ｉｇ Ａ 组对胃癌细胞具有促增殖作用，而此作用与血清胃泌素水平无明确关系。
To investigate the relationship between Helicobacter pylori (Hp) infection and the pathogenesis of gastric cancer. Serum samples of 49 patients diagnosed as gastric ulcer or duodenal ulcer by gastroscope and 20 normal healthy volunteers were collected. Serum gastrin levels were determined by radioimmunoassay (RIA) Serum anti-Hp Ig G, Ig A and Ig M levels, according to the results of serum antibody tests were divided into four groups observed serum on cultured human gastric cancer cells H A C803, human colon cancer cells H T29 proliferation, the cells Proliferation test using the M T T method. Results: Serum gastrin levels in patients with gastric and duodenal ulcer (139.6 ± 3817pg / m l) were significantly higher than those in normal people (63.12 ± 28.7pg / m l), with significant difference (P <0.01). High serum Hp Ig G antibody titers (group A) and high Hp Ig A antibody titers (group B) significantly enhanced the proliferation of human gastric cancer cells in patients with gastric and duodenal ulcer (P <0 05), especially the effect of high Hp Ig A antibody titer B group is more obvious. Serum of 4 groups of patients did not significantly promote the proliferation of human colon cancer cells (P> 0 05). Conclusion: Hp Ig G group and Hp Ig A group with high titer in serum of patients with stomach and duodenal ulcer infected with Hp can promote the proliferation of gastric cancer cells, and this effect has no clear relationship with serum gastrin level .