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以亚洲小车蝗为研究材料,提出了使用液氮保存蝗虫样本的有效方法,并用基因组DNA提取试剂盒分别对液氮速冻后保存、直接冷冻、无水乙醇保存和干制蝗虫标本进行了基因组DNA的提取和电泳检测。结果表明,在保存3个月后,检测蝗虫样本直接冷冻法和干标本提取的总DNA浓度较低,因而琼脂糖电泳检测亮度低;液氮速冻后保存和无水乙醇保存的蝗虫样本提取的基因组DNA浓度大,琼脂糖电泳检测亮度高。表明液氮速冻后保存和无水乙醇保存的标本适合用于基因组学研究。通过对比,直接冷冻保存与液氮速冻保存结果得出,蝗虫在冷冻胁迫死亡的过程中有DNA降解发生。
In this paper, we proposed an effective method for locust locust samples using liquid nitrogen, and genomic DNA was extracted from cryopreserved, frozen, ethanol-preserved and dried locust specimens using genomic DNA extraction kit Extraction and electrophoresis detection. The results showed that after 3 months of storage, the total DNA concentration of direct locust samples was lower than that of direct samples, so agarose electrophoresis showed low detection rate. The locust samples preserved in liquid nitrogen after freezing and ethanol preservation Genomic DNA concentration, agarose electrophoresis detection of high brightness. Samples that were stored frozen in liquid nitrogen and stored in absolute ethanol were suitable for genomic studies. By contrast, direct cryopreservation and liquid nitrogen cryopreservation results show that locusts die in the process of freezing stress DNA degradation occurs.