论文部分内容阅读
本研究以盲肠结扎穿孔（ＣＬＰ）方法制造腹腔感染模型，在ＣＬＰ前（ｎ＝８）、ＣＬＰ后４、８、１２、２４、４８和９６ｈ（ｎ＝６）分别处杀动物，并留取血和肝脏标本。用差别ＰＣＲ方法，建立了半定量检测肝脏白蛋白ｍＲＮＡ方法，初步观察到腹腔感染后４ｈ，肝脏白蛋白ｍＲＮＡ开始下降，感染后４８ｈ，白蛋白ｍＲＮＡ下降到正常对照的２７．２％，血浆白蛋白浓度与白蛋白ｍＲＮＡ密切相关（ｒ＝０．９２，Ｐ＜０．０１），提示腹腔感染早期白蛋白基因表达受到明显抑制，腹腔感染时低白蛋白血症与肝脏白蛋白ｍＲＮＡ表达下降有关。
In this study, the model of intraperitoneal infection was made by cecal ligation and puncture (CLP). Animals were killed before CLP (n = 8), 4, 8, 12, 24, 48 and 96 h after CLP (n = 6) Blood and liver specimens. Using differential PCR method, we established a method of semi-quantitative detection of hepatic albumin mRNA, initially observed 4h after intraperitoneal infection, liver albumin mRNA began to decline, 48h after infection, albumin mRNA decreased to 27.2% of normal control, plasma white The protein concentration was closely related to albumin mRNA (r = 0.92, P <0.01), which indicated that the expression of albumin gene in early stage of abdominal infection was significantly inhibited. Hypoproteinemia was associated with the decrease of hepatic albumin mRNA expression .