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在酸性溶液中，高锰酸钾能氧化核黄素产生化学发光，连二亚硫酸钠的存在可使发光强度大大增强。采用流动注射技术，建立了测定核黄素的化学发光分析法。方法的检出限为６．２×１０－８ｇ／ｍＬ，线性范围为２×１０－７～１×１０－５ｇ／ｍＬ，对４．０×１０－６ｇ／ｍＬ核黄素进行１１次平行测定，相对标准偏差为１．８％。方法用于针剂和片剂中核黄素测定，与药典标准方法－紫外分光光度法对照，相对误差不大于±２％。
In acidic solution, potassium permanganate can oxidize riboflavin to generate chemiluminescence, and the presence of sodium dithionite can greatly enhance the luminescence intensity. Flow injection technique was used to establish a chemiluminescence assay for the determination of riboflavin. The detection limit of this method was 6.2 × 10-8g / mL with a linear range of 2 × 10-7 ~ 1 × 10-5g / mL. Riboflavin of 4.0 × 10-6g / mL was parallelized 11 times The relative standard deviation was 1.8%. The method was applied to the determination of riboflavin in injection and tablet. Compared with the standard method of Pharmacopoeia - UV spectrophotometry, the relative error was no more than ± 2%.