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目的:建立并完善一种新的DNA(cDNA)检测方法,并探讨cDNA检测在鼻咽癌(nasopharyngealcarcinoma,NPC)患者诊断中的应用价值。方法:对196例经病理证实的NPC患者以微量基因组抽提试剂盒抽提血浆DNA,以荧光定量Realtime PCR测定含量,并以健康体检者125例作为对照组。结果:临床分期各组与治疗前相比(P均<0.05)差异有显著性;不论治疗前后,NPC患者各组与正常对照组相比,差异均有,(P均<0.05);cDNA含量与临床分期的关系:根据Ct值的逐渐减少,cDNA含量随着临床分期增加呈增高的趋势,但组间比较并无统计学意义(P>0.05)。结论:利用Realtime PCR法测定NPC患者cDNA,在NPC的诊断、疗效判断有着重要的临床应用价值。
Objective: To establish and perfect a new DNA (cDNA) detection method and to explore the value of cDNA detection in the diagnosis of nasopharyngeal carcinoma (NPC). Methods: Plasma DNA was extracted from 196 patients with pathologically confirmed NPC using Real-time PCR Kit by Real-time Quantitative Real-Time PCR. 125 healthy volunteers were used as control group. Results: There were significant differences in the clinical staging groups compared with those before treatment (all P <0.05). There was significant difference between the NPC group and the normal control group before and after treatment (P <0.05) Relationship with clinical stage: According to the gradual decrease of Ct value, the content of cDNA increased with the clinical stage, but there was no significant difference between the two groups (P> 0.05). Conclusion: Realtime PCR assay for NPC patients has important clinical value in the diagnosis and curative effect of NPC.