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目的分别合成T-2毒素、脱氧雪腐镰刀菌烯醇(DON)的全抗原。方法采用琥珀酸酐法对分子结构进行修饰,产生易反应的官能团,并与载体蛋白偶联,从而具备免疫条件,用高性能基质辅助激光解吸电离-飞行时间质谱仪MALDI-TOF检测偶联物及偶联蛋白的分子量,用商品化的T-2和DON免疫试剂盒与偶联物进行棋盘滴定验证。结果T-2和DON的全抗原及BSA的分子量分别为67641.6、66680.4、66297.7,偶联物与免疫试剂盒中T-2和DON的抗体均呈阳性反应。结论合成的T-2、DON全抗原为相应抗体的制备和免疫学方法的建立奠定了基础。
Objective To synthesize T-2 toxin and deoxynivalenol (DON) antigens respectively. Methods The molecular structure was modified by succinic anhydride method to generate reactive functional groups, which were coupled with the carrier protein to provide immunological conditions. The conjugates were detected by high performance matrix-assisted laser desorption / ionization-time of flight mass spectrometry (MALDI-TOF) The molecular weight of the coupled protein was verified by chessboard titration with a commercial T-2 and DON immunoassay kit. Results The total antigen of T-2 and DON and the molecular weight of BSA were 67641.6, 66680.4 and 66297.7, respectively. The conjugates showed positive reaction with antibodies to T-2 and DON in the immunization kit. Conclusion The synthetic T-2 and DON antigens provide the basis for the preparation of the corresponding antibodies and the establishment of immunological methods.