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为了通过测定蛋白质薄膜厚度变化而定量地研究生物分子间的相互作用 ,探讨了基于光学干涉法的薄膜厚度的测量方法。借助于在玻璃基底表面沉积的聚苯乙烯薄膜对噪声的抑制 ,使用非线性回归模型对生物传感器的检测信号进行了分析。通过反射干涉光谱法测定到乙型肝炎表面抗原在聚苯乙烯 -玻璃表面的吸附使薄膜厚度增加了3.3nm。随着5μg/ml,10μg/ml,20μg/ml,30μg/ml和50μg/ml浓度的乙型肝炎表面抗体的加入 ,薄膜厚度分别增加了3.7nm,4.3nm,5.3nm ,6.3nm和7.5nm。通过与经典的干涉测量技术和酶标记法相对照 ,证明了测量结果具有较高的可信度。
In order to quantitatively study the interaction between biomolecules by measuring the change of the thickness of the protein film, a method for measuring the thickness of the film based on the optical interference method is discussed. By means of the inhibition of the noise on the polystyrene film deposited on the glass substrate surface, the biosensor detection signal was analyzed using a non-linear regression model. The adsorption of Hepatitis B surface antigen on the polystyrene-glass surface by reflection interference spectroscopy increased the film thickness by 3.3 nm. With the addition of hepatitis B surface antibodies at concentrations of 5, 10, 20, 30 and 50 μg / ml, the film thickness increased by 3.7, 4.3, 5.3, 6.3 and 7.5 nm . By contrast with classical interferometry and enzyme labeling, it is demonstrated that the measurement results have high credibility.