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目的研究不同转移潜能非小细胞肺癌(NSCLC)细胞系中以及癌与癌旁组织中endoglin(ENG)的表达情况,探讨ENG在NSCLC的发生发展和转移复发中的作用。方法体外培养5株NSCLC细胞及1株正常支气管上皮细胞(HBE),随机选取22例NSCLC手术切除后癌与癌旁组织样本,分别采用real time PCR及Western blot法检测其中ENG mRNA和蛋白表达情况。采用χ2检验分析ENG的表达和临床资料之间的相关性。结果高转移性3株肺癌细胞系中ENG mRNA和蛋白表达显著升高,而正常细胞株及低转移性2株肺癌细胞中ENG表达缺失。22例肺癌样本中,其中19例癌组织中ENG mRNA和蛋白呈阳性表达(86.36%),且表达量明显高于相对应的癌旁组织(P<0.01)。ENG表达与患者临床病理特征年龄、性别、肿瘤大小、临床分期、病理分级、组织类型等均无关,而与患者淋巴结转移情况密切相关(P<0.01)。结论 ENG在NSCLC中的表达与淋巴结转移显著相关,有可能作为预测NSCLC转移及预后的分子标志。
Objective To investigate the expression of endoglin (ENG) in different metastatic potential non-small cell lung cancer (NSCLC) cell lines and adjacent tissues and to explore the role of ENG in the occurrence, development and metastasis of NSCLC. METHODS: Five NSCLC cells and one normal bronchial epithelial cell (HBE) were cultured in vitro. Twenty-two NSCLC specimens were collected from the cancer and para-cancerous tissues. Real-time PCR and Western blot were used to detect the mRNA and protein expression of ENG . Chi-square test was used to analyze the correlation between ENG expression and clinical data. Results The mRNA and protein expressions of ENG were significantly increased in the three highly metastatic lung cancer cell lines, but not in the normal and low metastatic lung cancer cells. Of the 22 lung cancer samples, 19 of them were positive for mRNA and protein expression of ENG (86.36%), and their expression levels were significantly higher than that of corresponding paracancerous tissues (P <0.01). The expression of ENG was not related to the clinicopathological features of patients, such as age, sex, tumor size, clinical stage, pathological grade, and histological type, but was closely related to lymph node metastasis (P <0.01). Conclusion The expression of ENG in NSCLC is significantly correlated with lymph node metastasis, which may serve as a molecular marker for predicting the metastasis and prognosis of NSCLC.