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用FeCl3溶液预处理蔗渣,运用Box-Behnken Design(BBD)设计,考察预处理的影响因子分别为:FeCl3预处理保温时间、反应温度、FeCl3浓度以及表面活性剂吐温80添加量,并对预处理蔗渣进行纤维素酶水解糖化。不同预处理条件对蔗渣各组分溶出及后续酶解产生显著影响。用傅立叶红外光谱(FTIR)分析了预处理前后化学结构变化。实验结果显示氯化铁溶液预处理能十分有效提高蔗渣酶解糖化效果,以总的葡聚糖产率为响应值优化预处理条件,得出较佳的预处理条件为:氯化铁浓度为0.05mol.L-1,吐温80用量1.00%(w/v),保温温度为180℃,保温时间为60min。
Pretreatment of bagasse with FeCl3 solution, using Box-Behnken Design (BBD) design, investigated the impact of pretreatment factors were: FeCl3 pretreatment holding time, reaction temperature, FeCl3 concentration and the amount of surfactant Tween 80 addition, and pre The bagasse is treated for cellulase hydrolysis saccharification. Different pretreatment conditions have significant effects on the dissolution of bagasse components and subsequent enzymolysis. The chemical structure before and after pretreatment was analyzed by Fourier transform infrared spectroscopy (FTIR). The experimental results show that ferric chloride solution pretreatment can effectively improve the saccharification efficiency of bagasse, optimize the pretreatment conditions with the total glucan yield as the response value, and obtain better pretreatment conditions as follows: the ferric chloride concentration is 0.05mol.L-1, Tween 80 dosage 1.00% (w / v), holding temperature 180 ℃, holding time 60min.