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根据ＧｅｎｅＢａｎｋ大鼠β－防御素－２ｃＤＮＡ序列，设计特异引物：Ｒ１５′３′ＧＣＡＣＣＡＧＧＣＴＴＣＡＧＴＣＡＴＧ；Ｒ２５′３′ＣＡＴＧＧＡＧＧＡＧＣＡＡＡＴＴＣＴＧ。引物由Ｇｉｂｃｏ公司合成。取Ｗｉｓｔａｒ大鼠宫颈组织，用Ｑｉａｇｅｎ公司生产的Ｒ
According to GeneBank rat β-defensin-2 cDNA sequence, design specific primers: R15’3’GCACCAGGCTTCAGTCATG; R25’3’CATGGAGGAGCAAATTCTG. Primers were synthesized by Gibco. Wistar rat cervical tissue was taken from Qiagen R