论文部分内容阅读
目的:研究雷帕霉素(RA)单独或联合阿霉素、顺铂及紫杉醇对子宫内膜癌细胞增殖和凋亡的影响,为临床应用RA治疗子宫内膜癌提供理论依据。方法:应用10μmol/L RA与各1/2的半数抑制浓度(IC50)量的阿霉素、顺铂及紫杉醇联合,细胞克隆形成法和流式法检测细胞存活率和凋亡率;RT-PCR法检测RA、阿霉素、顺铂及紫杉醇对细胞哺乳动物雷帕霉素靶蛋白(mTOR)mRNA表达影响;Western blot法检测10μmol/L RA作用6,12,24h后细胞Bcl-2蛋白表达水平。结果:RA联合化疗可显著提高各组化疗药的作用效果,降低细胞存活率,诱导细胞凋亡,与单纯化疗组相比有统计学差异(P<0.05);RA可显著降低宫颈癌细胞mTOR基因mRNA水平,抑制Bcl-2蛋白表达。结论:RA通过抑制mTOR通路,降低抗凋亡蛋白Bcl-2表达,提高化疗药物对子宫内膜癌细胞的杀伤作用。
Objective: To study the effects of rapamycin (RA) alone or in combination with doxorubicin, cisplatin and paclitaxel on the proliferation and apoptosis of endometrial cancer cells, providing a theoretical basis for the clinical application of RA in the treatment of endometrial cancer. Methods: Adriamycin, cisplatin and paclitaxel were used in combination with 10 μmol / L RA and each half of IC50. Cell viability and apoptosis were detected by cell clone formation and flow cytometry. RT- PCR was used to detect the effect of RA, doxorubicin, cisplatin and paclitaxel on the mRNA expression of mTOR in mammalian cells. Western blot was used to detect the expression of Bcl-2 protein at 6, 12 and 24 h after treated with 10 μmol / L RA The expression level. Results: RA combined with chemotherapy can significantly improve the effect of chemotherapy drugs, reduce cell survival and induce apoptosis, compared with the chemotherapy alone group (P <0.05); RA can significantly reduce the cervical cancer cells mTOR Gene mRNA level, inhibition of Bcl-2 protein expression. Conclusion: RA can decrease the expression of anti-apoptotic protein Bcl-2 by inhibiting the mTOR pathway and enhance the killing effect of chemotherapeutic drugs on endometrial cancer cells.