Magnetic starch particles(MSPs) were synthesized in water-in-oil microemulsion at room temperature.MSPs were characterized by transmission electron microscopy(TEM),Fourier transform infrared spectrometry(FTIR),zeta potential system,thermogravimetric analysis(TGA) and vibrating sample magnetometry(VSM).The average diameter of the MSPs was 220 nm,dispersed with well-proportioned size and magnetic resonance,the saturation magnetization was 3.64 A·m2/kg.MSP was coated with poly-L-lysine(PLL),and then the surface of PLL-MSP was combined with fluorescein isothiocynate(FITC).Results show that fluorescent/magnetic starch particles(FMSPs) are of stable photo-bleaching capability compared with free FITC,with low bio-toxicity and certain function of magnetic separation.It is expected that FMSPs are bifunctional nano-materials including fluorescence labelling and magnetic separation. Magnetic starch particles (MSPs) were synthesized in water-in-oil microemulsion at room temperature. MSPs were characterized by transmission electron microscopy (TEM), Fourier transform infrared spectrometry (FTIR), zeta potential system, thermogravimetric analysis (TGA) The average diameter of the MSPs was 220 nm, dispersed with well-proportioned size and magnetic resonance, the saturation magnetization was 3.64 A · m2 / kg. MSP was coated with poly-L-lysine (PLL), and then the surface of PLL-MSP was combined with fluorescein isothiocynate (FITC). Results show that fluorescent / magnetic starch particles (FMSPs) are of stable photo-bleaching capability compared with free FITC, with low bio-toxicity and certain function of magnetic separation .It is expected that FMSPs are bifunctional nano-materials including fluorescence labeling and magnetic separation.