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目的检测精子相关抗原9(SPAG9)在宫颈癌、宫颈上皮内瘤变(CIN)组织中的表达及意义。方法通过免疫组织化学、Western blotting、RT-PCR方法检测正常宫颈组织(对照组,n=20)、CIN组织(CIN组,n=35)及宫颈癌组织(宫颈癌组,n=40)中SPAG9的表达。应用酶联免疫吸附试验(ELISA)测定健康女性、子宫肌瘤患者、CIN患者及宫颈癌患者血清标本中SPAG9浓度。结果①SPAG9的表达:宫颈癌组>CIN组>对照组,各组间差异有统计学意义(P均<0.05);②SPAG9 mRNA和蛋白在宫颈癌组中高表达,在对照组中无表达(P<0.05);③宫颈癌患者的SPAG9血清浓度高于CIN患者、子宫肌瘤患者及健康女性的SPAG9血清浓度(P<0.05),且患者术后的SPAG9血清浓度下降。结论 SPAG9在宫颈癌和CIN组织以及患者血清中表达量均增高,其作为一个新的辅助生物学指标,可为宫颈癌及CIN的早期诊断、术后检测及随诊治疗提供指导意义。
Objective To detect the expression and significance of SPAG9 in cervical cancer and cervical intraepithelial neoplasia (CIN). Methods Immunohistochemistry, Western blotting and RT-PCR were used to detect the expression of VEGF in normal cervical tissue (control group, n = 20), CIN group (n = 35) and cervical cancer group SPAG9 expression. Serum SPAG9 concentrations in healthy women, uterine fibroids, CIN patients and cervical cancer patients were determined by enzyme-linked immunosorbent assay (ELISA). Results ① The expression of SPAG9 in cervical cancer group> CIN group> control group, the difference between each group was statistically significant (all P <0.05); ②SPAG9 mRNA and protein were highly expressed in cervical cancer group and no expression in control group (P < 0.05). (3) SPAG9 serum levels in patients with cervical cancer were higher than those in CIN patients, uterine fibroids patients and healthy women (P <0.05), and the SPAG9 serum concentration decreased after operation. Conclusions The expression of SPAG9 in cervical cancer and CIN tissues and in patients’ serum is increased. As a new auxiliary biological index, SPAG9 can be used as a guide for the early diagnosis, postoperative detection and follow-up of cervical cancer and CIN.