【摘 要】
：
ACCC 30150是由本实验室筛选的一株对黄瓜枯萎病、青椒疫病等多种土传病害具有较好防治效果的生防木霉菌.为了建立该生防木霉菌的CaCl2/PEG转化体系,对其原生质体制备及再生
【机 构】
：
东北农业大学资源与环境学院,中国农业科学院农业资源与农业区划研究所
论文部分内容阅读
ACCC 30150是由本实验室筛选的一株对黄瓜枯萎病、青椒疫病等多种土传病害具有较好防治效果的生防木霉菌.为了建立该生防木霉菌的CaCl2/PEG转化体系,对其原生质体制备及再生条件进行了摸索.结果表明,以0.6 M NaCl作为渗透压稳定剂,用PDA平板上培养36 h的微小菌丝接种液体菌丝培养基,菌龄16 h,用20 mg/ml的纤维素酶和蜗牛酶以1:1的比例混合,按照菌丝重量(g):酶液体积(ml)=1:20的比例,36℃酶解2 h原生质体产量最高.同时,酶解2 h,以CMR1为再生培养基原生质体的再生率最高.
其他文献
Background In-stent restenosis (ISR) has become one of the most challenging problems in patients with coronary heart disease. At present, using non-invasive met
Background Late incomplete stent apposition (ISA) may occur after drug-eluting stent implantation, affecting long-term clinical outcomes. This study aimed to ev
AIM:To assess the effect of notoginsenoside R1 on hepatic microcirculatory disturbance induced by gut ischemia/reperfusion (I/R) in mice. METHODS: The superior
背景与目的:探讨汉族、维族和哈族儿童δ-氨基-γ酮戊酸脱水酶(ALAD)、维生素D受体(VDR)基因多态性及其与铅中毒遗传易感性的关系. 材料与方法:采用聚合酶链式反应-限制性片
AIM:To detect the MLH1 gene promoter germlinemethylation in probands of Chinese hereditary nonpolyposis colorectal cancer (HNPCC),and to evaluate the role of me
AIM:To discuss the expression of glactin-3 in liver metastasis of colon cancer and its inhibition by modified citrus pectin (MCP) in mice.METHODS:Seventy-five B
Background The major difficulty in the research of DNA microarray data is the large number of genes compared with the relatively small number of samples as well
Background Urinary trypsin Inhibitor inhibits the enhanced production of pro-inflammatory molecules. Hemeoxygenase-1 induction protects against ischemia/reperfu
结论 在96 h的实验周期中,健康斑马鱼的K2Cr2O7 24 h-LC50为323.38 mg/L,Metlaomyl 96 h-LC50为4.91 mg/L;病鱼K2Cr2O724 h-LC50为302.55 mg/L,Methomyl 96 h-LC50 为3.04 mg
报道云南蝴蝶新纪录7种1型及订正种1种的简要形态特征、已知分布区和采集信息.其中新纪录包括凤蝶科1种1型,粉蝶科1种,眼蝶科2种,蛱蝶科1种,灰蝶科2种,订正种为灰蝶科1种.