采用室内好气培养方法,以DCD为参比对象研究了新型硝化抑制剂1-羟甲基-3,5-二甲基吡唑(DMHMP)对土壤硝化作用的影响。研究结果表明,DMHMP同DCD一样对土壤硝化作用有明显的抑制效应,主要表现在三个方面:(1)可使土壤NH4+-N含量在整个培养期内显著高于不添加抑制剂的对照处理(P<0.01);(2)使土壤NO3--N含量显著低于对照处理(P<0.01);(3)添加硝化抑制剂处理土壤的pH下降幅度和速度均较对照处理有所降低.当添加DMHMP的量与DCD相等时,其硝化抑制作用不如DCD,而当其添加量为DCD的2倍时,其硝化抑制效果明显优于DCD.在培养的第7天至第21天之间,DMHMP具有最优的硝化抑制效应。 Using indoor aerobic culture method, the effect of a new nitrification inhibitor, 1-hydroxymethyl-3,5-dimethylpyrazole (DMHMP), on soil nitrification was investigated using DCD as reference. The results showed that DMHMP had the same inhibitory effect on soil nitrification as DCD, which mainly manifested in three aspects: (1) The content of NH4 + -N in soil was significantly higher than that of control without adding inhibitor (P <0.01). (2) The content of NO3 - N in soil was significantly lower than that of the control (P <0.01). (3) The pH decrease and velocity of the soil treated with nitrification inhibitor were lower than the control. When the amount of DMHMP added is equal to that of DCD, its nitrification inhibition is not as good as that of DCD, and when it is added twice as much as DCD, its nitrification inhibitory effect is obviously better than that of DCD.In the seventh day to the 21st day of culture, , DMHMP has the best nitrification inhibitory effect.