论文部分内容阅读
目的:了解宁波地区伤寒和甲型副伤寒沙门菌的脉冲场凝胶电泳(PFGE)基因型和流行优势型,为探讨传播途径和追踪传染源等提供帮助。方法:用全自动细菌鉴定仪(Vitek32)确定细菌的生物学特征;用诊断血清确定细菌的血清型;用PFGE方法对细菌的染色体进行基因分型。结果:14株伤寒沙门菌分成3个PFGE基因型,364株甲型副伤寒沙门菌分成12个PFGE基因型,其中伤寒沙门菌1型10株,占71.43%,甲型副伤寒沙门菌2型295株,占81.04%;其次为1型42株,占11.54%;其他各型相对较少,伤寒沙门菌的流行优势型是1型,甲型副伤寒沙门菌是2型。菌株的带型变化在1~3条范围内,表明宁波地区的伤寒和甲型副伤寒沙门菌变异不大,有可能是同一克隆系的伤寒和甲型副伤寒沙门菌。结论:PFGE分型对探讨伤寒、副伤寒疫情内在联系具有重要意义,宁波地区的伤寒和甲型副伤寒疫情是本地菌株反复在人群带菌,污染环境和食物所致,应引起我们的关注。研究认为PFGE分型法特异性高、重复性好、结果容易判读,是一种分子分型的可信方法,为确定菌株之间的亲缘关系,提供了可靠的检测手段。
Objective: To understand the PFGE genotypes and epidemic predominance of typhoid fever and Paratyphoid Salmonella typhimurium in Ningbo, and to provide assistance in exploring the route of transmission and tracking the source of infection. Methods: The biological characteristics of the bacteria were determined by an automatic bacterial analyzer (Vitek32). The serotypes of the bacteria were determined by using the diagnostic serum. The genotypes of the bacteria were genotyped by PFGE. Results: 14 strains of Salmonella typhi were divided into 3 PFGE genotypes and 364 Salmonella paratyphi A strains were divided into 12 PFGE genotypes, of which 10 were Salmonella typhi type 1 (71.43%), Salmonella paratyphi type 2 295 strains, accounting for 81.04%; followed by 42 strains of type 1, accounting for 11.54%; other relatively less of each type, the prevalence of Salmonella typhi is type 1, Salmonella paratyphi type 2. Strains in the band-type changes in the range of 1 to 3, indicating that typhoid fever in Ningbo and Salmonella paratyphi A small variation may be the same clonal strains of Salmonella typhi and Salmonella typhi. CONCLUSION: PFGE typing is of great importance in exploring the intrinsic relationship between typhoid fever and paratyphoid fever. The epidemic situation of typhoid fever and paratyphoid fever in Ningbo is caused by repeated inoculation of local strains in the population, environmental pollution and food, which should be our concern. It is believed that the PFGE typing method is of high specificity, good repeatability and easy to interpret the results. It is a credible method of molecular typing and provides a reliable means of detection for determining the genetic relationship among strains.