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为探讨转化生长因子α(TGF-α)mRNA在大肠癌发生、发展中的作用及其临床意义,应用核酸杂交方法对14例大肠癌患者3组不同组织及直肠癌细胞系HR-8348进行TGF-αmRNA表达检测。结果显示:14例大肠癌3组不同组织及细胞系中均有TGF-αmRNA不同程度的表达,以癌远侧组织中表达为最弱:TGF-αmRNA表达与Dukes分期相关,可望作为判断大肠癌恶性程度的指标。另根据其在癌远侧组织中表达减弱趋势,提示临床是否可作为手术切缘的评估指标,尚有待于进一步的研究。
To investigate the role of transforming growth factor alpha (TGF-alpha) mRNA in the development and progression of colorectal cancer and the clinical significance, 14 cases of colorectal cancer patients with different colorectal cancer and rectal cancer cell line HR-8348 were treated with nucleic acid hybridization. -α mRNA expression assay. The results showed that TGF-α mRNA was expressed in different degrees in 14 different colorectal cancer tissues and cell lines. The expression of TGF-α mRNA was the weakest in the distal tissues of the cancer: the expression of TGF-α mRNA was related to Dukes stage, and it was expected to be a large intestine. The indicator of cancer malignancy. In addition, according to its weakened tendency in the distal tissues of cancer, whether it can be used as an evaluation index of surgical margins remains to be further studied.