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目的观察TNF-α和TNF-β在小鼠肝泡状棘球蚴病(简称泡球蚴病)中表达的动态变化,探讨小鼠肝泡球蚴病的免疫应答机制。方法选取3月龄雌性健康昆明小鼠,接种Em组织,建立小鼠原发性肝泡状棘球蚴模型,空白对照组用同样方法注射等量的生理盐水,以30d作为分隔点持续观察240d。收集小鼠血清,采用ELISA检测TNF-α和TNF-β浓度。结果与空白对照组相比,实验组小鼠血清TNF-α表达量于接种泡球蚴组织后30d开始增高,90d左右达到峰值,120d后逐渐降低;TNF-β表达量在接种泡球蚴组织后初期增长缓慢且维持较低水平,90d后开始明显增高,120d左右达到峰值,之后缓慢下降,但仍维持在较高水平。结论小鼠在感染肝泡状棘球蚴后TNF-α和TNF-β共同参与机体的免疫应答,且感染初期以TNF-α参与的细胞免疫为主,而当TNF-α开始降低时,TNF-β参与的体液免疫开始主导小鼠感染泡球蚴后的免疫应答机制。TNF-α和TNF-β既共同参与免疫应答,协同发挥作用,同时又相互制约,相互影响。
Objective To observe the dynamic changes of TNF-α and TNF-β expression in mice with alveolar hydatid disease (referred to as alveolar echinococcosis), and to explore the immune response mechanism of mice with alveolar echinococcosis. Methods Three-month-old female Kunming mice were inoculated with Em tissue to establish a mouse model of primary hepatic alveolar hydatid disease. The blank control group was injected with the same amount of saline by the same method and continued for 240 days . Mouse sera were collected and the concentrations of TNF-α and TNF-β were measured by ELISA. Results Compared with the blank control group, the expression of TNF-α in the experimental group began to increase 30 days after the inoculation of Echinococcus granulosus and peaked at 90 days, and gradually decreased after 120 days. The expression of TNF-β in the inoculated Echinococcus granulosus After the initial growth slow and maintain a low level, began to significantly increase after 90d, peaked around 120d, then slowly decreased, but still maintained at a high level. CONCLUSIONS: TNF-α and TNF-β are involved in the immune response in mice after infection with Echinococcus granulosus, and are predominantly involved in cellular immunity during the initial infection stage. When TNF-α begins to decrease, TNF The humoral immunity involved in β-β begins to dominate the immune response mechanism after infection of Cysticercus in mice. TNF-α and TNF-β are both involved in the immune response, synergistic effect, while restricting each other and influence each other.