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目的探索乙型重型肝炎患者HBV前C/BCP区变异与临床病情关系。方法用荧光定量PCR法检测血清HBVDNA载量;PCR扩增前C/BCP区基因后进行序列测定。结果30例乙型重型肝炎患者中BCP变异有18例,HB-VDNA平均水平为2.71×107copies/mL,未发生BCP变异者有12例,HBVDNA平均水平为5.35×106copies/mL,两者间有显著差异。测序结果显示HBV前C区nt1896G→A终止变异和BCP变异(nt1762A→T和1764G→A)在乙型重型肝炎患者中发生率分别为43.3%(13/30)和60%(18/30),在慢性乙型肝炎患者则分别为40%(12/30)和30%(9/30),两组BCP变异(nt1762A→T和1764G→A)相比有显著差异(P=0.02)。结论BCP变异可影响病毒复制,BCP变异可能与肝病的严重程度存在着相关性。
Objective To explore the relationship between HBV pre-C / BCP mutation and clinical conditions in patients with severe hepatitis B Methods Serum HBVDNA was detected by real-time PCR. Sequencing of the pre-C / BCP gene was performed by PCR. Results Among the 30 patients with severe hepatitis B, there were 18 cases of BCP variation, the average level of HB-VDNA was 2.71 × 107copies / mL, 12 cases without BCP mutation and the average level of HBVDNA was 5.35 × 106copies / mL Significant differences. The sequencing results showed that nt1896G → A termination mutation and BCP mutation (nt1762A → T and 1764G → A) in pre-HBV C region were 43.3% (13/30) and 60% (18/30) in patients with severe hepatitis B, respectively , And 40% (12/30) and 30% (9/30) respectively in patients with chronic hepatitis B. There was significant difference (P = 0.02) between two groups of BCP variation (nt1762A → T and 1764G → A). Conclusion BCP variation may affect virus replication, and BCP variation may be related to the severity of liver disease.